Difference between revisions of "Part:BBa K676004:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | Device constructed by performing 3A assembly between BBa_K676003 (TyrT promoter) and BBa_E0422 (RBA+eCFP+Ter). | + | Device constructed by performing 3A assembly between BBa_K676003 (TyrT promoter) and BBa_E0422 (RBA+eCFP+Ter) and pSB1C3 plasmid backbone. |
[[Image:UCL-Content-Experiment_Plan_for_Supercoilometer.jpg]] | [[Image:UCL-Content-Experiment_Plan_for_Supercoilometer.jpg]] | ||
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TyrT promoter from E coli genome and CFP expression casette from iGEM kit plate (Spring Distribution 2011 Kit Plate 1 - 8M). | TyrT promoter from E coli genome and CFP expression casette from iGEM kit plate (Spring Distribution 2011 Kit Plate 1 - 8M). | ||
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Latest revision as of 21:07, 1 October 2011
Supercoilometer
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Device constructed by performing 3A assembly between BBa_K676003 (TyrT promoter) and BBa_E0422 (RBA+eCFP+Ter) and pSB1C3 plasmid backbone.
Source
TyrT promoter from E coli genome and CFP expression casette from iGEM kit plate (Spring Distribution 2011 Kit Plate 1 - 8M).