Difference between revisions of "Part:BBa K544003"
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<partinfo>BBa_K544003 short</partinfo> | <partinfo>BBa_K544003 short</partinfo> | ||
− | + | A transcriptional regulator TetR (BBa_C0040) is driven by constitutive promoter family members (BBa-J23103, BBa-J23109, BBa-J23116, and BBa-J23106), which can be used to tune the expression level of constitutively expressed TetR. | |
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
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<partinfo>BBa_K544003 SequenceAndFeatures</partinfo> | <partinfo>BBa_K544003 SequenceAndFeatures</partinfo> | ||
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+ | <div ALIGN=CENTER> | ||
+ | {| style="width:254px;background:#99EE63;text-align:center;font-family: georgia, helvetica, arial, sans-serif;color:#000000;margin- top:5px;padding: 2px;" cellspacing="5"; | ||
+ | |- | ||
+ | |[[Image:Table 1.jpg|450px]] | ||
+ | |- | ||
+ | |Fluorescent intensity of MG1655 -tetO2-0-sfGFP strain after transformation of various repressors | ||
+ | |} | ||
+ | </div> | ||
+ | |||
+ | |||
+ | |||
+ | (The data collected are considered only when difference between values with the P-value <0.05) | ||
+ | |||
+ | |||
+ | |||
+ | <div ALIGN=CENTER> | ||
+ | {| style="width:254px;background:#99EE63;text-align:center;font-family: georgia, helvetica, arial, sans-serif;color:#000000;margin- top:5px;padding: 2px;" cellspacing="5"; | ||
+ | |- | ||
+ | |[[Image:Table 2.jpg|450px]] | ||
+ | |- | ||
+ | |Fluorescent intensity of MG1655 -tetO2-0-EGFP strain after transformation of various repressors | ||
+ | |} | ||
+ | </div> | ||
+ | |||
+ | |||
+ | (The data collected are considered only when difference between values with the P-value <0.05) | ||
+ | |||
+ | [EGFP: Enhanced Green Fluorescent Protein, sfGFP: Superfolder Green Fluorescent protein ] | ||
<!-- Uncomment this to enable Functional Parameter display | <!-- Uncomment this to enable Functional Parameter display |
Latest revision as of 19:23, 5 October 2011
Tetracycline Repressor with Consitutive Promoter BBa_J23116
A transcriptional regulator TetR (BBa_C0040) is driven by constitutive promoter family members (BBa-J23103, BBa-J23109, BBa-J23116, and BBa-J23106), which can be used to tune the expression level of constitutively expressed TetR.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 65
Illegal SpeI site found at 37 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal SpeI site found at 37 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 65
Illegal SpeI site found at 37 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 65
Illegal SpeI site found at 37 - 1000COMPATIBLE WITH RFC[1000]
(The data collected are considered only when difference between values with the P-value <0.05)
(The data collected are considered only when difference between values with the P-value <0.05)
[EGFP: Enhanced Green Fluorescent Protein, sfGFP: Superfolder Green Fluorescent protein ]