Difference between revisions of "Part:BBa K559010"

Latest revision as of 22:29, 14 October 2011

Halorhodopsin complete system

Halorhodopsin gene,light-driven ion pump, allow chloride ion to be pumped in by the light input. The mechanism can be refered to BBa_K559000.

Halorhodopsin gene is under regulation by constitute T7 promoter to allow protein coding to be maximized. The details can be refered to BBa_K559001.

Bidirectional terminator is added for high efficiency termination of both forward and backward transcription

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 368
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 380
Illegal AgeI site found at 106
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 316

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K559010 short</partinfo>
-Halorhodopsin gene allow chloride ion to be pumped in by the level of light intensity. Halorhodopsin gene is under regulation by constitute T7 promoter to allow protein coding to be maximized, and the bidirectional terminator is added for high efficiency termination of both forward and backward transcription
+Halorhodopsin gene,light-driven ion pump, allow chloride ion to be pumped in by the light input. The mechanism can be refered to <partinfo>BBa_K559000</partinfo>.
+Halorhodopsin gene is under regulation by constitute T7 promoter to allow protein coding to be maximized. The details can be refered to <partinfo>BBa_K559001</partinfo>.
+Bidirectional terminator is added for high efficiency termination of both forward and backward transcription
 <!-- Add more about the biology of this part here
 ===Usage and Biology===
+This is the complete functional system that we use in iGEM CUHK 11 team project '''Chloricolight'''.
+The characterization and the applications can be found in 'Experience' part.
 <!-- -->