Difference between revisions of "Part:BBa K646003:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | Using parts BBa_K646000 and BBa_I719005, a T7 promoted Hemeoxygenase was created by digesting the T7 biobrick with SpeI and PstI and ligating it to the HO insert digested with XbaI and PstI. This procedure can be used if an EcoRI and PstI cut plasmid backbone is not available. | |
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| + | Successful expression in E.coli should result in green colonies due to the presence of biliverdin (synthesized by heme oxygenase 1). | ||
===Source=== | ===Source=== | ||
Latest revision as of 13:22, 30 September 2011
T7 promoter with Hemeoxygenase
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Using parts BBa_K646000 and BBa_I719005, a T7 promoted Hemeoxygenase was created by digesting the T7 biobrick with SpeI and PstI and ligating it to the HO insert digested with XbaI and PstI. This procedure can be used if an EcoRI and PstI cut plasmid backbone is not available.
Successful expression in E.coli should result in green colonies due to the presence of biliverdin (synthesized by heme oxygenase 1).
Source
BBa_K646000 and BBa_I719005