Difference between revisions of "Part:BBa K574006:Design"
(→Design Notes) |
|||
(One intermediate revision by the same user not shown) | |||
Line 1: | Line 1: | ||
− | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K574006 short</partinfo> | <partinfo>BBa_K574006 short</partinfo> | ||
Line 7: | Line 6: | ||
===Design Notes=== | ===Design Notes=== | ||
− | + | We design this part to regulate the sending of the 3OC12HSL. L-arabinose acts as an inducer which we can add into the system and TetR acts as a repressor which comes from the system inside. | |
− | + | ||
− | + | ||
===Source=== | ===Source=== | ||
− | + | We used BioBrick Standard Assembly. | |
− | + | List as follows: | |
+ | ''<partinfo>R0040</partinfo>'' | ||
+ | ''<partinfo>K081016</partinfo>'' | ||
+ | ''<partinfo>I13453</partinfo>'' | ||
+ | ''<partinfo>K081009</partinfo>'' | ||
+ | ''<partinfo>E0430</partinfo>'' | ||
===References=== | ===References=== |
Latest revision as of 14:31, 4 October 2011
3OC12HSL regulated by pTet and pBad
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 930
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 744
Illegal BglII site found at 1625
Illegal BamHI site found at 870 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 306
Illegal AgeI site found at 1187 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We design this part to regulate the sending of the 3OC12HSL. L-arabinose acts as an inducer which we can add into the system and TetR acts as a repressor which comes from the system inside.
Source
We used BioBrick Standard Assembly. List as follows: BBa_R0040 BBa_K081016 BBa_I13453 BBa_K081009 BBa_E0430