Difference between revisions of "Part:BBa K646000:Design"

 
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<partinfo>BBa_K646000 short</partinfo>
 
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===Design Notes===
 
===Design Notes===
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The gene was digested with X and S before ligation, several ligations were done to ensure correct orientation.
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The gene was digested with XbaI and SpeI for the BioBrick construction due to the primer design, and the gene orientation was screened by an XbaI digest of the constructed BioBrick. For subsequent usage of this part, iGEM BioBrick assembly protocol could be used; digest using either EcoRI with SpeI or XbaI with PstI.
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===Source===
 
===Source===

Latest revision as of 12:47, 30 September 2011

Hemeoxygenase with RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The gene was digested with XbaI and SpeI for the BioBrick construction due to the primer design, and the gene orientation was screened by an XbaI digest of the constructed BioBrick. For subsequent usage of this part, iGEM BioBrick assembly protocol could be used; digest using either EcoRI with SpeI or XbaI with PstI.

Source

Http://www.ncbi.nlm.nih.gov/gene/953483

References