Difference between revisions of "Part:BBa J176014"

Latest revision as of 00:06, 30 September 2011

Intron

3 exons & 2 introns; intron 2 has SIBR cassette for Bbsl insertion of miRNA

Usage and Biology

This BioBrick is used for adding introns to the messenger RNA of a gene. Introns are thought to enhance the expression of genes in mammalian cells, although introns aren't absolutely necessary for mammalian synthetic genes. This part is also handy for co-expressing a microRNA of your own choice (Chung et al., 2006) along with a visible reporter protein. When using this part, place it between the promoter and the translation start+coding sequence of your gene.

REFERENCES:

Chung KH, Hart CC, Al-Bassam S, Avery A, Taylor J, Patel PD, Vojtek AB, Turner DL. (2006) Polycistronic RNA polymerase II expression vectors for RNA interference based on BIC/miR-155. NAR. 34:e53. PMID: 16614444

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1176
Illegal XhoI site found at 1253
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 139

@@ Line 7: / Line 7: @@
 ===Usage and Biology===
-This BioBrick is used for adding introns to a coding sequence. Introns are thought to enhance the expression of genes in mammalian cells, although introns aren't absolutely necessary for synthetic genes. This part is also handy for co-expressing a microRNA of your own choice (Chung et al., 2006) along with a visible reporter protein. When using this part, place it between the promoter and the start codon/Kozak+coding sequence of your gene.
+This BioBrick is used for adding introns to the messenger RNA of a gene. Introns are thought to enhance the expression of genes in mammalian cells, although introns aren't absolutely necessary for mammalian synthetic genes. This part is also handy for co-expressing a microRNA of your own choice (Chung et al., 2006) along with a visible reporter protein. When using this part, place it between the promoter and the translation start+coding sequence of your gene.
 REFERENCES:
 <!-- Last FN, Last2 FN2. (year) Title. Journal abbrev. Vol:p1-p100. -->
-# FChung KH, Hart CC, Al-Bassam S, Avery A, Taylor J, Patel PD, Vojtek AB, Turner DL. (2006) Polycistronic RNA polymerase II expression vectors for RNA interference based on BIC/miR-155. NAR. 34:e53. PMID: 16614444
+# Chung KH, Hart CC, Al-Bassam S, Avery A, Taylor J, Patel PD, Vojtek AB, Turner DL. (2006) Polycistronic RNA polymerase II expression vectors for RNA interference based on BIC/miR-155. NAR. 34:e53. PMID: 16614444
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