Difference between revisions of "Part:BBa J176025"

 
 
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<partinfo>BBa_J176025 short</partinfo>
 
<partinfo>BBa_J176025 short</partinfo>
  
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miRNA target for luciferase RNAi
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miRNA target for luciferase (artificial RNAi system)
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<br>
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===Usage and Biology===
 
===Usage and Biology===
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* Put this sequence in the 3' UTR of a gene to place it under the control of miR-luc
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This sequence contains 5 repeats that have partial complementarity with an artificial microRNA (from firefly luciferase gene). This target site is intended to mimic the natural microRNA silencing pathway (as opposed to siRNA silencing). The luciferase RNAi system is orthogonal to mammalian cell RNAi pathways, so it should not have any cross-talk with natural miRNAs/ targets.
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Latest revision as of 03:05, 11 December 2011

miRts luc

miRNA target for luciferase (artificial RNAi system)



Usage and Biology

  • Put this sequence in the 3' UTR of a gene to place it under the control of miR-luc


This sequence contains 5 repeats that have partial complementarity with an artificial microRNA (from firefly luciferase gene). This target site is intended to mimic the natural microRNA silencing pathway (as opposed to siRNA silencing). The luciferase RNAi system is orthogonal to mammalian cell RNAi pathways, so it should not have any cross-talk with natural miRNAs/ targets.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 21
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]