Difference between revisions of "Part:BBa J176014"
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<partinfo>BBa_J176014 short</partinfo>
 
<partinfo>BBa_J176014 short</partinfo>
  
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3 exons & 2 introns; intron 2 has SIBR cassette for Bbsl insertion of miRNA (Chung NAR 2006)
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3 exons & 2 introns; intron 2 has SIBR cassette for Bbsl insertion of miRNA
  
 
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===Usage and Biology===
 
===Usage and Biology===
  
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This BioBrick is used for adding introns to the messenger RNA of a gene. Introns are thought to enhance the expression of genes in mammalian cells, although introns aren't absolutely necessary for mammalian synthetic genes. This part is also handy for co-expressing a microRNA of your own choice (Chung et al., 2006) along with a visible reporter protein. When using this part, place it between the promoter and the translation start+coding sequence of your gene.
  
 
REFERENCES:
 
REFERENCES:
 
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# FChung KH, Hart CC, Al-Bassam S, Avery A, Taylor J, Patel PD, Vojtek AB, Turner DL. (2006) Polycistronic RNA polymerase II expression vectors for RNA interference based on BIC/miR-155. NAR. 34:e53. PMID: 16614444
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# Chung KH, Hart CC, Al-Bassam S, Avery A, Taylor J, Patel PD, Vojtek AB, Turner DL. (2006) Polycistronic RNA polymerase II expression vectors for RNA interference based on BIC/miR-155. NAR. 34:e53. PMID: 16614444
  
 
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Latest revision as of 00:06, 30 September 2011

Intron

3 exons & 2 introns; intron 2 has SIBR cassette for Bbsl insertion of miRNA

Usage and Biology

This BioBrick is used for adding introns to the messenger RNA of a gene. Introns are thought to enhance the expression of genes in mammalian cells, although introns aren't absolutely necessary for mammalian synthetic genes. This part is also handy for co-expressing a microRNA of your own choice (Chung et al., 2006) along with a visible reporter protein. When using this part, place it between the promoter and the translation start+coding sequence of your gene.

REFERENCES:

  1. Chung KH, Hart CC, Al-Bassam S, Avery A, Taylor J, Patel PD, Vojtek AB, Turner DL. (2006) Polycistronic RNA polymerase II expression vectors for RNA interference based on BIC/miR-155. NAR. 34:e53. PMID: 16614444

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1176
    Illegal XhoI site found at 1253
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 139