Difference between revisions of "Part:BBa J176014"
(→‎Usage and Biology)
 
(7 intermediate revisions by the same user not shown)
Line 2: Line 2:
 
<partinfo>BBa_J176014 short</partinfo>
 
<partinfo>BBa_J176014 short</partinfo>
  
−
3 exons & 2 introns; intron 2 has SIBR cassette for Bbsl insertion of miRNA (Chung NAR 2006)
+
3 exons & 2 introns; intron 2 has SIBR cassette for Bbsl insertion of miRNA
  
−
<!-- Add more about the biology of this part here
+
<!-- Add more about the biology of this part here -->
 
===Usage and Biology===
 
===Usage and Biology===
  
−
REFERENCES:<!-- Last FN, Last2 FN2. (year) Title. Journal abbrev. Vol:p1-p100. -->
+
This BioBrick is used for adding introns to the messenger RNA of a gene. Introns are thought to enhance the expression of genes in mammalian cells, although introns aren't absolutely necessary for mammalian synthetic genes. This part is also handy for co-expressing a microRNA of your own choice (Chung et al., 2006) along with a visible reporter protein. When using this part, place it between the promoter and the translation start+coding sequence of your gene.
−
# FChung KH, Hart CC, Al-Bassam S, Avery A, Taylor J, Patel PD, Vojtek AB, Turner DL. (2006) Polycistronic RNA polymerase II expression vectors for RNA interference based on BIC/miR-155. NAR. 34:e53. PMID: 16614444
+
 
 +
REFERENCES:
 +
<!-- Last FN, Last2 FN2. (year) Title. Journal abbrev. Vol:p1-p100. -->
 +
# Chung KH, Hart CC, Al-Bassam S, Avery A, Taylor J, Patel PD, Vojtek AB, Turner DL. (2006) Polycistronic RNA polymerase II expression vectors for RNA interference based on BIC/miR-155. NAR. 34:e53. PMID: 16614444
  
 
<!-- -->
 
<!-- -->

Latest revision as of 00:06, 30 September 2011

Intron

3 exons & 2 introns; intron 2 has SIBR cassette for Bbsl insertion of miRNA

Usage and Biology

This BioBrick is used for adding introns to the messenger RNA of a gene. Introns are thought to enhance the expression of genes in mammalian cells, although introns aren't absolutely necessary for mammalian synthetic genes. This part is also handy for co-expressing a microRNA of your own choice (Chung et al., 2006) along with a visible reporter protein. When using this part, place it between the promoter and the translation start+coding sequence of your gene.

REFERENCES:

  1. Chung KH, Hart CC, Al-Bassam S, Avery A, Taylor J, Patel PD, Vojtek AB, Turner DL. (2006) Polycistronic RNA polymerase II expression vectors for RNA interference based on BIC/miR-155. NAR. 34:e53. PMID: 16614444

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1176
    Illegal XhoI site found at 1253
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 139