Difference between revisions of "Part:BBa J176013:Experience"
(→Pc-TF chromatin-based transcription factor) |
|||
(6 intermediate revisions by the same user not shown) | |||
Line 1: | Line 1: | ||
− | |||
__NOTOC__ | __NOTOC__ | ||
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
Line 5: | Line 4: | ||
===Applications of BBa_J176013=== | ===Applications of BBa_J176013=== | ||
+ | |||
+ | ==Pc-TF chromatin-based transcription factor== | ||
+ | |||
+ | I used this activation domain to create a synthetic modular mammalian transcription factor that binds to a DNA-asscoatied protein instead of to the DNA itself. See hPCD-TF (<partinfo>BBa_J176062</partinfo>) for details. [[User:Kahaynes|Kahaynes]] | ||
===User Reviews=== | ===User Reviews=== | ||
<!-- DON'T DELETE --><partinfo>BBa_J176013 StartReviews</partinfo> | <!-- DON'T DELETE --><partinfo>BBa_J176013 StartReviews</partinfo> | ||
− | <!-- Template for a user review | + | |
+ | {|width='80%' style='border:1px solid gray' | ||
+ | |- | ||
+ | |width='10%'| | ||
+ | <partinfo>BBa_J176013 AddReview 5</partinfo> | ||
+ | <I>Karmella Haynes</I> | ||
+ | |width='60%' valign='top'| | ||
+ | '''VP64 activity is sensitive to promoter proximity.'''<br> | ||
+ | This is an "oops" discovery that I first learned about from Tom Armel and Jason Lohmeuller (P.A. Silver lab), then reproduced through improving one of my failed inducible promoters. In my hands, VP64 activity is maximized when it is targeted to DNA sequences that are ~40-80 base pairs away from a minimal promoter. When fused to the Gal4 DNA binding domain (<partinfo>BBa_J176020</partinfo>) and targeted to Gal (UAS) DNA elements (<partinfo>BBa_J176019</partinfo>) that are assembled immediately adjacent to the HSVtkTATA minimal promoter (<partinfo>BBa_J176011</partinfo>), I observed weak activation, and perhaps repression, of a fluorescent reporter gene. In contrast, when a small spacer (<partinfo>BBa_J176039</partinfo>) is inserted between the Gal4 binding sites and the promoter, reporter gene activity greatly increases. (Data will be posted soon) | ||
+ | |} | ||
+ | |||
+ | |||
+ | <!-- Template for a user review. Copy and paste this code before editing | ||
{|width='80%' style='border:1px solid gray' | {|width='80%' style='border:1px solid gray' | ||
|- | |- | ||
Line 15: | Line 30: | ||
<I>Username</I> | <I>Username</I> | ||
|width='60%' valign='top'| | |width='60%' valign='top'| | ||
− | Enter the review | + | Enter the review information here. |
|}; | |}; | ||
<!-- End of the user review template --> | <!-- End of the user review template --> | ||
<!-- DON'T DELETE --><partinfo>BBa_J176013 EndReviews</partinfo> | <!-- DON'T DELETE --><partinfo>BBa_J176013 EndReviews</partinfo> |
Latest revision as of 21:18, 18 February 2012
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_J176013
Pc-TF chromatin-based transcription factor
I used this activation domain to create a synthetic modular mammalian transcription factor that binds to a DNA-asscoatied protein instead of to the DNA itself. See hPCD-TF (BBa_J176062) for details. Kahaynes
User Reviews
UNIQ2a939393e6781226-partinfo-00000001-QINU
•••••
Karmella Haynes |
VP64 activity is sensitive to promoter proximity. |
UNIQ2a939393e6781226-partinfo-00000007-QINU