Difference between revisions of "Part:BBa K543015"

Latest revision as of 16:07, 5 October 2011

TetR generator (J23100-B0031-R0040-B0015)

TetR generator (J23100-B0031-R0040-B0015).

Repress expression of GFP by Anti-killing device

We confirmed whether Anti-killing Device is working or not. We transform Anti-killing Device into E.coli which have GFP generator(I13522) and observed its florescence. Figure is a result of this experiment. Left is E.coli transformed Anti-killing Device, right is control. we confirmed Anti-killing Device represseｓ GFP expression weakly.
Need more experience to get quantitative date.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K543015 short</partinfo>
 TetR generator (J23100-B0031-R0040-B0015).
+[[Image:Tokyo_Metropolitan_Killing2.jpg|right|250px]]
+'''Repress expression of GFP by Anti-killing device'''<br>
+We confirmed whether Anti-killing Device is working or not. We transform Anti-killing Device into E.coli which have GFP generator(I13522) and observed its florescence. Figure is a result of this experiment. Left is E.coli transformed Anti-killing Device, right is control. we confirmed Anti-killing Device represseｓ GFP expression weakly.<br>
+Need more experience to get quantitative date. <br>
 <!-- Add more about the biology of this part here
 ===Usage and Biology===
 <!-- -->
 <span class='h3bb'>Sequence and Features</span>