Difference between revisions of "Part:BBa K672001:Design"
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===Source=== | ===Source=== | ||
| − | + | [[Part:BBa_K410000]] (hybB-ompA-AOX1A)<br> | |
| − | + | [[Part:BBa_K328001]] (CsPA promoter) | |
===References=== | ===References=== | ||
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*Hankins JS., Zappavigna C., Prud'homme-Genereux A., Mackie GA., 2007, Role of RNA structure and susceptibility to RNase E in regulation of a cold shock mRNA, cspA mRNA., J Bacteriol. 189(12):4353-8<br> | *Hankins JS., Zappavigna C., Prud'homme-Genereux A., Mackie GA., 2007, Role of RNA structure and susceptibility to RNase E in regulation of a cold shock mRNA, cspA mRNA., J Bacteriol. 189(12):4353-8<br> | ||
*Xia B., Ke H., Jiang W., Inouye M., 2002, The Cold Box stem-loop proximal to the 5'-end of the Escherichia coli cspA gene stabilizes its mRNA at low temperature., J Biol Chem. 277(8):6005-11 | *Xia B., Ke H., Jiang W., Inouye M., 2002, The Cold Box stem-loop proximal to the 5'-end of the Escherichia coli cspA gene stabilizes its mRNA at low temperature., J Biol Chem. 277(8):6005-11 | ||
| − | == Aox == | + | == OmpA + Aox == |
*Grant, N et al. “Two Cys or Not Two Cys? That is the Question; Alternative Oxidase in the Thermogenic Plant sacred Lotus.” Plant Physiology 150 (2009): 987-995. {PAPER} | *Grant, N et al. “Two Cys or Not Two Cys? That is the Question; Alternative Oxidase in the Thermogenic Plant sacred Lotus.” Plant Physiology 150 (2009): 987-995. {PAPER} | ||
*OmpA Sequence:[[http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Datasheet/e8283dat.Par.0001.File.tmp/e8283dat.pdf]] | *OmpA Sequence:[[http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Datasheet/e8283dat.Par.0001.File.tmp/e8283dat.pdf]] | ||
*AoxA Sequence:[[http://www.ncbi.nlm.nih.gov/nuccore/226897260#sequence_226897260]] | *AoxA Sequence:[[http://www.ncbi.nlm.nih.gov/nuccore/226897260#sequence_226897260]] | ||
Latest revision as of 07:34, 29 September 2011
New Promoter For Periplasmic Heat Generation
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Unknown
- 12INCOMPATIBLE WITH RFC[12]Unknown
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Unknown
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
As the part K328000 is a basic part, instead of a combination of subparts (Promoter, etc...), we had to analyse the sequence, cut out the HybB promoter and combine manually with the CsPA sequence.
Source
Part:BBa_K410000 (hybB-ompA-AOX1A)
Part:BBa_K328001 (CsPA promoter)
References
CsPA Promoter
- Fang L., Jiang W., Bae W., Inouye M., 1997, Promoter-independent cold-shock induction of cspA and its derepression at 37 degrees C by mRNA stabilization., Mol Microbiol. 23(2):355-64
- Hankins JS., Zappavigna C., Prud'homme-Genereux A., Mackie GA., 2007, Role of RNA structure and susceptibility to RNase E in regulation of a cold shock mRNA, cspA mRNA., J Bacteriol. 189(12):4353-8
- Xia B., Ke H., Jiang W., Inouye M., 2002, The Cold Box stem-loop proximal to the 5'-end of the Escherichia coli cspA gene stabilizes its mRNA at low temperature., J Biol Chem. 277(8):6005-11
OmpA + Aox
- Grant, N et al. “Two Cys or Not Two Cys? That is the Question; Alternative Oxidase in the Thermogenic Plant sacred Lotus.” Plant Physiology 150 (2009): 987-995. {PAPER}
- OmpA Sequence:http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Datasheet/e8283dat.Par.0001.File.tmp/e8283dat.pdf
- AoxA Sequence:http://www.ncbi.nlm.nih.gov/nuccore/226897260#sequence_226897260