Difference between revisions of "Part:BBa K511201"
 
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This part encodes a protein that combines the Mnt DNA-binding domain with the VP16 viral transactivation domain. This synthetic transactivator is able to moderately activate expression from minCMV-Mnt promoter variants.
 
This part encodes a protein that combines the Mnt DNA-binding domain with the VP16 viral transactivation domain. This synthetic transactivator is able to moderately activate expression from minCMV-Mnt promoter variants.
  
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For more information, consult the MIT iGEM 2011 wiki <html><a href="http://2011.igem.org/Team:MIT/Results">here.</a></html>
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<html><img src='https://static.igem.org/mediawiki/2011/4/4c/Mnt_Characterization.jpg
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' style="width:60%"><br></html>
  
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For more information, consult the MIT iGEM 2011 wiki <html><a href="http://2011.igem.org/Team:MIT/Results">here.</a></html>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 04:11, 29 September 2011

Mnt-VP16 Transactivator MammoBlock

This part encodes a protein that combines the Mnt DNA-binding domain with the VP16 viral transactivation domain. This synthetic transactivator is able to moderately activate expression from minCMV-Mnt promoter variants.


For more information, consult the MIT iGEM 2011 wiki here.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 93
    Illegal PstI site found at 106
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 93
    Illegal PstI site found at 106
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 93
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 93
    Illegal PstI site found at 106
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 93
    Illegal PstI site found at 106
  • 1000
    COMPATIBLE WITH RFC[1000]