Difference between revisions of "Part:BBa K524001:Design"

 
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__NOTOC__
 
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<partinfo>BBa_K524001 short</partinfo>
 
<partinfo>BBa_K524001 short</partinfo>
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===Design Notes===
 
===Design Notes===
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A stop codon was added to the end of the CDS to terminate translation of sfGFP1-10
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A stop codon was added to the end of the CDS to terminate translation of sfGFP1-10.
−
 
+
RBS was introduced into primers for cloning.
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+
  
 
===Source===
 
===Source===
  
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Constructed from 2011 iGEM DNA Repository Plates and Boxes, Spring Distribution, BBa_I746908
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Constructed from 2011 iGEM DNA Repository Plates and Boxes, Spring Distribution, [https://parts.igem.org/Part:BBa_I746908 BBa_I746908]
  
 
===References===
 
===References===
 +
 +
Stéphanie Cabantous, Thomas C Terwilliger & Geoffrey S Waldo.(2004).Protein tagging and detection with engineered self-assembling fragments of green fluorescent protein.Nature Biotechnology 23, 102 - 107
 +
 +
Stéphanie Cabantous & Geoffrey S Waldo.(2006).''In vivo'' and ''in vitro'' protein solubility assays using split GFP.Nature Methods - 3, 845 - 854
 +
 +
Jun Zhou, Jian Lin, Cuihong Zhou, Xiaoyan Deng and Bin Xia.(2011).An improved bimolecular fluorescence complementation tool based on superfolder green fluorescent protein.Acta Biochim Biophys Sin 43 (3): 239-244.

Latest revision as of 14:24, 5 October 2011

pLac + RBS + split sfGFP 1-10


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 238


Design Notes

A stop codon was added to the end of the CDS to terminate translation of sfGFP1-10. RBS was introduced into primers for cloning.

Source

Constructed from 2011 iGEM DNA Repository Plates and Boxes, Spring Distribution, BBa_I746908

References

Stéphanie Cabantous, Thomas C Terwilliger & Geoffrey S Waldo.(2004).Protein tagging and detection with engineered self-assembling fragments of green fluorescent protein.Nature Biotechnology 23, 102 - 107

Stéphanie Cabantous & Geoffrey S Waldo.(2006).In vivo and in vitro protein solubility assays using split GFP.Nature Methods - 3, 845 - 854

Jun Zhou, Jian Lin, Cuihong Zhou, Xiaoyan Deng and Bin Xia.(2011).An improved bimolecular fluorescence complementation tool based on superfolder green fluorescent protein.Acta Biochim Biophys Sin 43 (3): 239-244.