Difference between revisions of "Part:BBa K511826"

 
(rollbackEdits.php mass rollback)
 
(2 intermediate revisions by one other user not shown)
(No difference)

Latest revision as of 16:18, 10 May 2013


Inducible N-Cadherin-2A-EYFP Generator (UAS-Gal4-N-Cadherin-2A-EYFP) MammoBlock Device

This MammoBlock composite device produces both the N-Cadherin cell-cell adhesion compound and the yellow fluorescent protein EYFP when induced with Gal4 transactivator variants and otherwise produces both proteins at a low, basal (OFF) level. This generator is particularly well suited to the engineering of cellular patterns given the combination of adhesion and fluorescent readout.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 126
    Illegal SpeI site found at 2965
    Illegal PstI site found at 529
    Illegal PstI site found at 1630
    Illegal PstI site found at 3241
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 126
    Illegal NheI site found at 154
    Illegal SpeI site found at 2965
    Illegal PstI site found at 529
    Illegal PstI site found at 1630
    Illegal PstI site found at 3241
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 126
    Illegal BamHI site found at 1162
    Illegal XhoI site found at 83
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 126
    Illegal SpeI site found at 2965
    Illegal PstI site found at 529
    Illegal PstI site found at 1630
    Illegal PstI site found at 3241
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 126
    Illegal SpeI site found at 2965
    Illegal PstI site found at 529
    Illegal PstI site found at 1630
    Illegal PstI site found at 3241
    Illegal NgoMIV site found at 1481
    Illegal NgoMIV site found at 3007
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1516
    Illegal BsaI.rc site found at 2073