Difference between revisions of "Part:BBa K566027"
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<partinfo>BBa_K566027 short</partinfo> | <partinfo>BBa_K566027 short</partinfo> | ||
| − | Ccas is a member of cyanobacteriochrome family of proteins. Phycocyanobillin (PCB) binds at a conserved cysteine domain of CcaS and activates signaling activity. CcaS, along with its response regulator CcaR, activates promoter pCpcG2. | + | Ccas is a member of cyanobacteriochrome family of proteins. Phycocyanobillin (PCB) binds at a conserved cysteine domain of CcaS and activates signaling activity. CcaS, along with its response regulator CcaR, activates transcription of promoter pCpcG2. |
Activation shows max response with 535 nm wavelength green light and with 672 nm wavelength red light. When absorbing green light, CcaS autophosphorylates and then carries out phosphotransfer to CcaR. Phosphorylated CcaR (CcaR*) acts as a transcription factor for pCpcG2. This process can be reverted with absorption of red light. | Activation shows max response with 535 nm wavelength green light and with 672 nm wavelength red light. When absorbing green light, CcaS autophosphorylates and then carries out phosphotransfer to CcaR. Phosphorylated CcaR (CcaR*) acts as a transcription factor for pCpcG2. This process can be reverted with absorption of red light. | ||
Latest revision as of 00:19, 29 September 2011
CcaS
Ccas is a member of cyanobacteriochrome family of proteins. Phycocyanobillin (PCB) binds at a conserved cysteine domain of CcaS and activates signaling activity. CcaS, along with its response regulator CcaR, activates transcription of promoter pCpcG2. Activation shows max response with 535 nm wavelength green light and with 672 nm wavelength red light. When absorbing green light, CcaS autophosphorylates and then carries out phosphotransfer to CcaR. Phosphorylated CcaR (CcaR*) acts as a transcription factor for pCpcG2. This process can be reverted with absorption of red light.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1235
Illegal SpeI site found at 1330 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1235
Illegal NheI site found at 606
Illegal SpeI site found at 1330 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1235
Illegal XhoI site found at 2252 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1235
Illegal SpeI site found at 1330 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1235
Illegal SpeI site found at 1330
Illegal AgeI site found at 1270 - 1000COMPATIBLE WITH RFC[1000]