Difference between revisions of "Part:BBa K511818"

 
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Latest revision as of 16:18, 10 May 2013


Inducible Notch-Gal4-ESN Receptor Generator (TRE-Tight-Notch-Gal4-ESN) MammoBlock Device

This MammoBlock composite device produces the Notch-Gal4-ESN juxtacrine signaling receptor when induced with tTA/rtTA transactivator variants in the presence of tetracycline analogues, and otherwise produces Notch-Gal4-ESN at a low, basal (OFF) level. This device is applicable to cell patterning experiments employing the Notch-Delta system and is generally useful for the interrogation or engineering of multicellular phenomena that rely upon cell-cell contact.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal XbaI site found at 30
    Illegal PstI site found at 602
    Illegal PstI site found at 1007
    Illegal PstI site found at 1337
    Illegal PstI site found at 1568
    Illegal PstI site found at 1665
    Illegal PstI site found at 2855
    Illegal PstI site found at 4733
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal PstI site found at 602
    Illegal PstI site found at 1007
    Illegal PstI site found at 1337
    Illegal PstI site found at 1568
    Illegal PstI site found at 1665
    Illegal PstI site found at 2855
    Illegal PstI site found at 4733
    Illegal NotI site found at 5568
    Illegal NotI site found at 5576
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal BamHI site found at 3277
    Illegal XhoI site found at 5859
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal XbaI site found at 30
    Illegal PstI site found at 602
    Illegal PstI site found at 1007
    Illegal PstI site found at 1337
    Illegal PstI site found at 1568
    Illegal PstI site found at 1665
    Illegal PstI site found at 2855
    Illegal PstI site found at 4733
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal XbaI site found at 30
    Illegal PstI site found at 602
    Illegal PstI site found at 1007
    Illegal PstI site found at 1337
    Illegal PstI site found at 1568
    Illegal PstI site found at 1665
    Illegal PstI site found at 2855
    Illegal PstI site found at 4733
    Illegal NgoMIV site found at 868
    Illegal NgoMIV site found at 2980
    Illegal NgoMIV site found at 3036
    Illegal NgoMIV site found at 3079
    Illegal NgoMIV site found at 3864
    Illegal NgoMIV site found at 4881
    Illegal NgoMIV site found at 5058
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 5778
    Illegal BsaI.rc site found at 437
    Illegal BsaI.rc site found at 2145
    Illegal BsaI.rc site found at 3060
    Illegal BsaI.rc site found at 5517