Difference between revisions of "Part:BBa K653001:Design"

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<partinfo>BBa_K653001 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K653001 SequenceAndFeatures</partinfo>
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===Design Notes===
 
===Design Notes===
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The assembling of this two parts was performed using the 3A Assembly Method.
 
The assembling of this two parts was performed using the 3A Assembly Method.
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===Source===
 
===Source===
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The [https://parts.igem.org/Part:BBa_B0015 BBa_B0015 Double Terminator part] is the most commonly used terminator. It seems to be reliable.
 
The [https://parts.igem.org/Part:BBa_B0015 BBa_B0015 Double Terminator part] is the most commonly used terminator. It seems to be reliable.
 +
 
Note, however, that Part:BBa_B0014 is a better part for forward and reverse termination.  
 
Note, however, that Part:BBa_B0014 is a better part for forward and reverse termination.  
 
The part was designed by Reshma Shetty
 
The part was designed by Reshma Shetty
  
 
===References===
 
===References===
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<biblio>
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#Elowitz pmid=10659856
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#Andersen pmid=9603842
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#Valdivia pmid=8707053
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#Double Terminator by Reshma Shetty [https://parts.igem.org/Part:BBa_B0015 BBa_B0015]
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</biblio>

Latest revision as of 22:57, 28 September 2011

GFP reporter plus Double Terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 644

Design Notes

This GFP part was derived from the reporter device used by Elowitz's repressilator : GFPmut3* has a couple of mutations from wtGFP - S2R and S65G and S72A. Emerald and EGFP have S65T and multiple other mutations. The S2R mutation should have no effect.

The Double terminator consisting of BBa_B0010 and BBa_B0012. This is the most commonly used terminator. It seems to be reliable.

The assembling of this two parts was performed using the 3A Assembly Method.

Source

This BBa_E0040part was derived from the reporter device used by Elowitz's repressilator [1]. Elowitz obtained the GFP tagged sequence (gfpmut3*) published by Andersen and coworkers [2] to build the reporter device. Andersen constructed those GFP variants based on a gfpmut3b containing plasmid that was a gift from R. H. Valdivia [3]. The PCR method used in construction by Andersen changed aa residue 2 from serine to arginine with no detectable phenotypic effect in their experiments.

The BBa_B0015 Double Terminator part is the most commonly used terminator. It seems to be reliable.

Note, however, that Part:BBa_B0014 is a better part for forward and reverse termination. The part was designed by Reshma Shetty

References

<biblio>

  1. Elowitz pmid=10659856
  2. Andersen pmid=9603842
  3. Valdivia pmid=8707053
  4. Double Terminator by Reshma Shetty BBa_B0015


</biblio>