Difference between revisions of "Part:BBa K590026"
 
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This part consists of [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590031 Aldehyde Decarbonylase], from ''Synechococcus elongatus'', in PSB1C3 high consititutive vector.  This was an important negative control in testing [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590025 the PetroBrick] - which consists of Aldehyde Decarbonylase and another part from ''Synechococcus elongatus'', [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590027 Acyl-ACP Reductase].
 
This part consists of [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590031 Aldehyde Decarbonylase], from ''Synechococcus elongatus'', in PSB1C3 high consititutive vector.  This was an important negative control in testing [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590025 the PetroBrick] - which consists of Aldehyde Decarbonylase and another part from ''Synechococcus elongatus'', [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590027 Acyl-ACP Reductase].
  
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
  
 
[[Image:Fatty_Acid_Biosynthesis.jpg|right|frameless|300px]]
 
[[Image:Fatty_Acid_Biosynthesis.jpg|right|frameless|300px]]
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This part produces ADC, which is the second step in the conversion of cellular fatty acyl-ACPs to alkanes.  ADC decarbonylates the aldehyde produced in the first step by Acyl-ACP Reductase.  This part is best used in combination with some kind of reductase, to form alkanes from the aldehydes made by the reductase.
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This part produces ADC, which catalyzes the second step in the conversion of cellular fatty acyl-ACPs to alkanes.  ADC decarbonylates the aldehyde produced in the first step by Acyl-ACP Reductase.  This part is best used in combination with some kind of reductase, to form alkanes from the aldehydes made by the reductase.
  
 
[[Image:Washinton_AARADCGC-MS.png|left|400px|thumb|GC plot showing that alkanes are only produced when both ADC and AAR are expressed.  The blue peak corresponds to ADC alone - which only generates alkanes when sufficient aldehyde (produced by a cellular reductase) is present.]]
 
[[Image:Washinton_AARADCGC-MS.png|left|400px|thumb|GC plot showing that alkanes are only produced when both ADC and AAR are expressed.  The blue peak corresponds to ADC alone - which only generates alkanes when sufficient aldehyde (produced by a cellular reductase) is present.]]
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K590026 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K590026 SequenceAndFeatures</partinfo>

Latest revision as of 20:11, 28 September 2011

ADC-PSB1C3-High consititutive

This part consists of Aldehyde Decarbonylase, from Synechococcus elongatus, in PSB1C3 high consititutive vector. This was an important negative control in testing the PetroBrick - which consists of Aldehyde Decarbonylase and another part from Synechococcus elongatus, Acyl-ACP Reductase.

Usage and Biology

Fatty Acid Biosynthesis.jpg

This part produces ADC, which catalyzes the second step in the conversion of cellular fatty acyl-ACPs to alkanes. ADC decarbonylates the aldehyde produced in the first step by Acyl-ACP Reductase. This part is best used in combination with some kind of reductase, to form alkanes from the aldehydes made by the reductase.

GC plot showing that alkanes are only produced when both ADC and AAR are expressed. The blue peak corresponds to ADC alone - which only generates alkanes when sufficient aldehyde (produced by a cellular reductase) is present.





















Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 470
    Illegal NheI site found at 493
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1254
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 126
  • 1000
    COMPATIBLE WITH RFC[1000]