Difference between revisions of "Part:BBa K542004"

Latest revision as of 01:59, 29 September 2011

pLacI Regulated Lumazine Synthase with Transciptional Terminator and pBAD

Made by assembling BBa_K542001 with BBa_K542003.

Note that the pLacI promoter (BBa_R0010) is regulating the Lumazine Synthase gene, not the pBAD promoter (BBa_I13453) which is downstream. Therefore, Lumazine Synthase production is IPTG inducible.
The microcompartment portion to the "Co-localization Construct". (BBa_K542008)

This part was characterized in BBa_K542008 in E. coli strain DH5alpha to demonstrate its functionality as an intermediate in that construct. The data sheet for BBa_K542008 is shown below.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 961
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 901
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 <br><br>
 Note that the pLacI promoter ([https://parts.igem.org/wiki/index.php?title=Part:BBa_R0010 BBa_R0010]) is regulating the Lumazine Synthase gene, not the pBAD promoter ([https://parts.igem.org/Part:BBa_I13453 BBa_I13453]) which is downstream. Therefore, Lumazine Synthase production is IPTG inducible. <br>
-The microcompartment portion to the "Co-localization Construct".
+The microcompartment portion to the "Co-localization Construct". ([https://parts.igem.org/Part:BBa_K542008 BBa_K542008])
 <br><br>
-Datasheet for the test construct <html><a href="https://parts.igem.org/Part:BBa_K542008"><font color="blue">Part BBa_K542008</font></a></html> in <i>E. coli</i> strain DH5alpha demonstrating this is a functional intermediate in that construct.
+This part was characterized in <html><a href="https://parts.igem.org/Part:BBa_K542008"><font color="blue">BBa_K542008</font></a></html> in <i>E. coli</i> strain DH5alpha to demonstrate its functionality as an intermediate in that construct. The data sheet for BBa_K542008 is shown below.
 <br><br>
 [[image:uoflECdatasheet.png|150px]]