Difference between revisions of "Part:BBa K598008"
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<partinfo>BBa_K598008 short</partinfo> | <partinfo>BBa_K598008 short</partinfo> | ||
− | 1.20 | + | This is a basic part that consists of thiamine pyrophosphate (TPP) ligand responsive hammerhead ribozyme numbered 2.5 with native RBS (AAGGAGAT), BBa_E0040 and BBa_B0015.(fig.1) |
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+ | [[Image:PekingR fp.png|center|thumb|600px| '''Figure 1''' Construction of TPP UP-regulated Hammerhead Ribozyme 1.20 with Native RBS+E0040+B0015. This part consists of TPP ribozyme 1.20 with native RBS (AAGGAGAT), BBa_E0040 and BBa_B0015.It is obtained by PCR from TPP-HHAz 1.20 [1] which is kindly provided by Markus Wieland ''et al.'', and then inserted into pSB1C3 through standard assembly. | ||
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+ | This part is obtained by PCR from TPP-HHAZ 1.20 [1] which is kindly provided by Markus Wieland et. al., and then inserted into pSB1C3 through standard assembly. | ||
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+ | == Reference == | ||
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+ | [1] Markus Wieland, Armin Benz, Benedikt Klauser, and Jörg S. Hartig. (2009). Artificial Ribozyme Switches Containing Natural Riboswitch Aptamer Domains. Angew. Chem. 121, 2753-2756 | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Latest revision as of 20:02, 5 October 2011
TPP Up-regulated Hammerhead Ribozyme 1.20 with Native RBS+E0040+B0015
This is a basic part that consists of thiamine pyrophosphate (TPP) ligand responsive hammerhead ribozyme numbered 2.5 with native RBS (AAGGAGAT), BBa_E0040 and BBa_B0015.(fig.1)
This part is obtained by PCR from TPP-HHAZ 1.20 [1] which is kindly provided by Markus Wieland et. al., and then inserted into pSB1C3 through standard assembly.
Reference
[1] Markus Wieland, Armin Benz, Benedikt Klauser, and Jörg S. Hartig. (2009). Artificial Ribozyme Switches Containing Natural Riboswitch Aptamer Domains. Angew. Chem. 121, 2753-2756
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 794