Difference between revisions of "Part:BBa K566007"

Latest revision as of 06:35, 28 September 2011

PenI repressor optimized for E. coli (inverted sequence)

Optimized PenI repressor with LVA tag from Bacillus licheniformis, with preferential codon usage for improved expression in E. coli. PenI negatively regulates transcription from pPenI promoter (BBa_R0074).

Usage and Biology

The penicillinase repressor (PenI) negatively regulates expression of the penicillinase gene (penP) in Bacillus licheniformis by binding to its operators located within the promoter region of penP. penI codes for a protein with 128 amino acids. Filter-binding analyses suggest that the active form of the repressor is a dimer.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K566010 short</partinfo>
-Optimized PenI repressor with LVA tag from ''Bacillus licheniformis'', with preferential codon usage for improved expression in <i>E. coli</i>. Negatively regulates transcription from pPenI promoter ([https://parts.igem.org/Part:BBa_R0074 BBa_R0074]).
+Optimized PenI repressor with LVA tag from ''Bacillus licheniformis'', with preferential codon usage for improved expression in <i>E. coli</i>. PenI negatively regulates transcription from pPenI promoter ([https://parts.igem.org/Part:BBa_R0074 BBa_R0074]).
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+The penicillinase repressor (PenI) negatively regulates expression of the penicillinase gene (penP) in <i>Bacillus licheniformis</i> by binding to its operators located within the promoter region of penP. penI codes for a protein with 128 amino acids. Filter-binding analyses suggest that the active form of the repressor is a dimer.
-<!-- -->
 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K566010 SequenceAndFeatures</partinfo>