Difference between revisions of "Part:BBa K566014"

Latest revision as of 05:06, 28 September 2011

pRM-RBS-cI434

Composite part for Biphasic switch tests. Include pRM promoter from phage λ pRM promoter from Lambda phage. It may be positively and negatively regulated according to cI protein concentration. Low [cI] turn it ON, high [cI] turn it OFF. It contains three operators named OR1, OR2 and OR3, all cI binding sites. When bound to OR1 and OR2, cI positively regulates pRM; cI binding to OR3 repress pRM. However, due to a low binding affinity to OR3, higher concentrations of the protein are required.

A medium RBS sequence, strength is considered relative to Strong (BBa_B0030), Weak (BBa_B0031) and weaker (BBa_B0033). Also contain the cI434 coding sequence optimized for E. coli, its come from the registered part cI434 repressor protein (BBa_C0052), It binds to the 434 regulatory sequence in λ pRM promoter. The sequence contains a LVA tag for faster degradation.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 534
Illegal AgeI site found at 545
1000
COMPATIBLE WITH RFC[1000]

Revision as of 05:03, 28 September 2011 (view source) Arrius (Talk \| contribs) ← Older edit		Latest revision as of 05:06, 28 September 2011 (view source) Arrius (Talk \| contribs)
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	Composite part for Biphasic switch tests. Include pRM promoter from phage λ pRM promoter from Lambda phage. It may be positively and negatively regulated according to cI protein concentration. Low [cI] turn it ON, high [cI] turn it OFF. It contains three operators named OR1, OR2 and OR3, all cI binding sites. When bound to OR1 and OR2, cI positively regulates pRM; cI binding to OR3 repress pRM. However, due to a low binding affinity to OR3, higher concentrations of the protein are required.		Composite part for Biphasic switch tests. Include pRM promoter from phage λ pRM promoter from Lambda phage. It may be positively and negatively regulated according to cI protein concentration. Low [cI] turn it ON, high [cI] turn it OFF. It contains three operators named OR1, OR2 and OR3, all cI binding sites. When bound to OR1 and OR2, cI positively regulates pRM; cI binding to OR3 repress pRM. However, due to a low binding affinity to OR3, higher concentrations of the protein are required.

−	A medium RBS sequence, strength is considered relative to Strong (<bbpart>BBa_B0030</bbpart>), Weak (BBa_B0031) and weaker (BBa_B0033). Also contain the cI434 coding sequence optimized for ''E. coli'', its come from the registered part cI434 repressor protein, It binds to the 434 regulatory sequence in λ pRM promoter. The sequence contains a LVA tag for faster degradation.	+	A medium RBS sequence, strength is considered relative to Strong (<bbpart>BBa_B0030</bbpart>), Weak (<bbpart>BBa_B0031</bbpart>) and weaker (<bbpart>BBa_B0033</bbpart>). Also contain the cI434 coding sequence optimized for ''E. coli'', its come from the registered part cI434 repressor protein (<bbpart>BBa_C0052</bbpart>), It binds to the 434 regulatory sequence in λ pRM promoter. The sequence contains a LVA tag for faster degradation.