Difference between revisions of "Part:BBa K566012"
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Preferential codons to improve their expression in ''E. coli''. | Preferential codons to improve their expression in ''E. coli''. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
+ | The lambda repressor is a dimeric protein also known as the cI protein. Lambda cI protein has the capability to bind several operators within a complex regulatory region that involves mainly three promoters (pRM, pR and pL). There are two well-known sets of cI binding sites in lambda (OR and OL) spaced 2.4 kb apart and composed of three operators each (OR1:OR2:OR3; OL1:OL2:OL3) (figure 1). cI has a higher affinity for OR1 and OR2 operators, where binding positively regulates the pRM promoter. On the other hand, when bound to OR3 cI represses the promoter; however, due to a low binding affinity, a higher concentration of the protein is required. Thus, cI can act as both an activator -at low concentrations- and a repressor -at high concentrations- on pRM. Modified with a LVA tag for rapid degradation of the protein. | ||
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+ | [[Image:CI.png|thumb|center|600px|Figure 1. The λ operators OL and OR. The promoter pRM transcribes cI, rexA, and rexB in a lysogen, which sum up to size of ∼2.4 Kb. | ||
+ | Figure taken and modified from Court DL et al. (2007) <i>J Bacteriol</i> 189:298–304. | ||
+ | .]] | ||
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Latest revision as of 22:49, 28 September 2011
cI repressor from Lambda phage optimized for E. coli cI (+LVA)
cI repressor from Lambda phage optimized for E. coli. Includes LVA tag.
Preferential codons to improve their expression in E. coli.
Usage and Biology
The lambda repressor is a dimeric protein also known as the cI protein. Lambda cI protein has the capability to bind several operators within a complex regulatory region that involves mainly three promoters (pRM, pR and pL). There are two well-known sets of cI binding sites in lambda (OR and OL) spaced 2.4 kb apart and composed of three operators each (OR1:OR2:OR3; OL1:OL2:OL3) (figure 1). cI has a higher affinity for OR1 and OR2 operators, where binding positively regulates the pRM promoter. On the other hand, when bound to OR3 cI represses the promoter; however, due to a low binding affinity, a higher concentration of the protein is required. Thus, cI can act as both an activator -at low concentrations- and a repressor -at high concentrations- on pRM. Modified with a LVA tag for rapid degradation of the protein.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 463
- 1000COMPATIBLE WITH RFC[1000]