Difference between revisions of "Part:BBa K518010:Experience"

 
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===User Reviews===
 
===User Reviews===
 
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<partinfo>BBa_K518010 AddReview number</partinfo>
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<partinfo>BBa_K518010 AddReview 5</partinfo>
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<I>Username</I>
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<I>Cooper Union iGEM 2014</I>
 
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Enter the review inofrmation here.
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We used this part to design a composite part, <partinfo>BBa_K1354000</partinfo>, with this promoter and a phage activator, <partinfo>BBa_I746352</partinfo>. This phage activator turned on a phage promoter with a GFP reporter composite part, <partinfo>BBa_I746321</partinfo>. The two composite parts were in separate plasmids. When shocked with UV, the UV promoter was turned on, which activated the phage activator. This then turned the phage promoter on which activated the GFP reporter. The fluorescence readings are shown below.
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[[File:CU_UV_promoter_graph.JPG]]
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[[File:CU_UV_promoter_table.JPG]]
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Latest revision as of 18:54, 15 October 2014


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K518010

User Reviews

UNIQ9650083d09b60ef5-partinfo-00000000-QINU

•••••

Cooper Union iGEM 2014

We used this part to design a composite part, BBa_K1354000, with this promoter and a phage activator, BBa_I746352. This phage activator turned on a phage promoter with a GFP reporter composite part, BBa_I746321. The two composite parts were in separate plasmids. When shocked with UV, the UV promoter was turned on, which activated the phage activator. This then turned the phage promoter on which activated the GFP reporter. The fluorescence readings are shown below. CU UV promoter graph.JPG CU UV promoter table.JPG

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UNIQ9650083d09b60ef5-partinfo-00000005-QINU