Difference between revisions of "Part:BBa K566010"
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<partinfo>BBa_K566010 short</partinfo> | <partinfo>BBa_K566010 short</partinfo> | ||
| − | Optimized PenI repressor with LVA tag from Bacillus licheniformis, with preferential codon usage for improved expression in E. coli. Negatively regulates transcription from pPenI promoter ([https://parts.igem.org/Part:BBa_R0074 BBa_R0074]). The nucleotide sequence was inverted to ease DNA synthesis. | + | Optimized PenI repressor with LVA tag from ''Bacillus licheniformis'', with preferential codon usage for improved expression in <i>E. coli</i>. Negatively regulates transcription from pPenI promoter ([https://parts.igem.org/Part:BBa_R0074 BBa_R0074]). The nucleotide sequence was inverted to ease DNA synthesis. |
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===Usage and Biology=== | ===Usage and Biology=== | ||
| + | The penicillinase repressor (PenI) negatively regulates expression of the penicillinase gene (penP) in <i>Bacillus licheniformis</i> by binding to its operators located within the promoter region of penP. penI codes for a protein with 128 amino acids. Filter-binding analyses suggest that the active form of the repressor is a dimer. | ||
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Latest revision as of 07:12, 28 September 2011
PenI repressor optimized for E. coli (inverted sequence)
Optimized PenI repressor with LVA tag from Bacillus licheniformis, with preferential codon usage for improved expression in E. coli. Negatively regulates transcription from pPenI promoter (BBa_R0074). The nucleotide sequence was inverted to ease DNA synthesis.
Usage and Biology
The penicillinase repressor (PenI) negatively regulates expression of the penicillinase gene (penP) in Bacillus licheniformis by binding to its operators located within the promoter region of penP. penI codes for a protein with 128 amino acids. Filter-binding analyses suggest that the active form of the repressor is a dimer.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]