Difference between revisions of "Part:BBa K569017:Design"
(→Source) |
|||
| (One intermediate revision by the same user not shown) | |||
| Line 1: | Line 1: | ||
| − | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K569017 short</partinfo> | <partinfo>BBa_K569017 short</partinfo> | ||
| Line 7: | Line 6: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | + | Used primers: ccatctagacacctgcaaaaggagatgg and ccaactagtcacctgcaaaaagggacat to PCR CheY from K422007 from ETHZ_Basel 2010. We added in the correct BioBrick restriction enzymes in the PCR and placed it into the pSB1C3 backbone. | |
| − | + | ||
| − | + | ||
===Source=== | ===Source=== | ||
| − | + | K422007 ETHZ_Basel 2010 | |
| − | + | ||
===References=== | ===References=== | ||
Latest revision as of 16:28, 28 September 2011
CheY
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 275
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 275
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 275
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 275
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Used primers: ccatctagacacctgcaaaaggagatgg and ccaactagtcacctgcaaaaagggacat to PCR CheY from K422007 from ETHZ_Basel 2010. We added in the correct BioBrick restriction enzymes in the PCR and placed it into the pSB1C3 backbone.
Source
K422007 ETHZ_Basel 2010