Difference between revisions of "Part:BBa K566015:Design"
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− | Michalowski CB, Short MD, Little JW (2004) Sequence tolerance of the phage λ PRM promoter: implications for evolution of gene regulatory circuitry <i>J Bacteriol</i> <b>186</b>:7899-7999. | + | <b>1.</b>Michalowski CB, Short MD, Little JW (2004) Sequence tolerance of the phage λ PRM promoter: implications for evolution of gene regulatory circuitry <i>J Bacteriol</i> <b>186</b>:7899-7999. |
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Latest revision as of 16:30, 27 September 2011
pRM-RBS-cI434
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 547
Illegal AgeI site found at 558 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Search for illegal sites was performed, they were found and deleted.
Source
Synthesized DNA.
References
1.Michalowski CB, Short MD, Little JW (2004) Sequence tolerance of the phage λ PRM promoter: implications for evolution of gene regulatory circuitry J Bacteriol 186:7899-7999.