Difference between revisions of "Part:BBa K566023:Design"
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===References=== | ===References=== | ||
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<b>1.</b> Tabor JJ, Levskaya A, Voigt CA (2010) Multichromatic Control of Gene Expression in <i>Escherichia coli</i>. <i>J Mol Biol</i> <b>405</b>:315-324. | <b>1.</b> Tabor JJ, Levskaya A, Voigt CA (2010) Multichromatic Control of Gene Expression in <i>Escherichia coli</i>. <i>J Mol Biol</i> <b>405</b>:315-324. | ||
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− | </ | + | <b>2.</b> Fukuyama K. <i>et al</i> (2010) Structural Insights into Vinyl Reduction Regiospecificity of Phycocyanobilin:Ferredoxin Oxidoreductase (PcyA). <i>J Biol Chem.</i> <b>285</b>(2): 1000–1007.</p> |
Latest revision as of 01:21, 29 September 2011
pcyA gene (constitutive expression)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 386
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
For the project that this part was made, it was needed to use other Assambly Standard.
Source
CDS was extracted by PCR, promoter and terminator were Classic BioBricks.
References
1. Tabor JJ, Levskaya A, Voigt CA (2010) Multichromatic Control of Gene Expression in Escherichia coli. J Mol Biol 405:315-324.
2. Fukuyama K. et al (2010) Structural Insights into Vinyl Reduction Regiospecificity of Phycocyanobilin:Ferredoxin Oxidoreductase (PcyA). J Biol Chem. 285(2): 1000–1007.