Difference between revisions of "Part:BBa K598003:Design"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K598003 short</partinfo> | <partinfo>BBa_K598003 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
− | + | This part is a thiamine pyrophosphate (TPP) ligand responsive ribozyme numbered 2.5 with native RBS (AAGGAGAT), which can inhibit gene expression responding to TPP. Although there are standard restriction sites in this part, attaching upstream of target gene can only be operated by site-directed mutation. Because the spacing between RBS and start code of target gene after standard assembly is not appropriate. | |
Line 13: | Line 12: | ||
===Source=== | ===Source=== | ||
− | + | This part is obtained by PCR from inactive TPP-HHAZ 2.5 which is kindly provided by Markus Wieland et. al., and then inserted into pSB1C3 through standard assembly. | |
− | + | ||
===References=== | ===References=== | ||
+ | Markus Wieland, Armin Benz, Benedikt Klauser, and Jörg S. Hartig. (2009). Artificial Ribozyme Switches Containing Natural Riboswitch Aptamer Domains. Angew. Chem. 121, 2753-2756 |
Latest revision as of 08:54, 5 October 2011
TPP Down-regulated Hammerhead Ribozyme 2.5 with Native RBS
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 121
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part is a thiamine pyrophosphate (TPP) ligand responsive ribozyme numbered 2.5 with native RBS (AAGGAGAT), which can inhibit gene expression responding to TPP. Although there are standard restriction sites in this part, attaching upstream of target gene can only be operated by site-directed mutation. Because the spacing between RBS and start code of target gene after standard assembly is not appropriate.
Source
This part is obtained by PCR from inactive TPP-HHAZ 2.5 which is kindly provided by Markus Wieland et. al., and then inserted into pSB1C3 through standard assembly.
References
Markus Wieland, Armin Benz, Benedikt Klauser, and Jörg S. Hartig. (2009). Artificial Ribozyme Switches Containing Natural Riboswitch Aptamer Domains. Angew. Chem. 121, 2753-2756