Difference between revisions of "Part:BBa K598017:Design"

 
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<partinfo>BBa_K598017 short</partinfo>
 
<partinfo>BBa_K598017 short</partinfo>
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===Design Notes===
 
===Design Notes===
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keep the specificity of the aptamer and the effectivity of the hammerhead
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This part constructs by connecting adenine aptamer from ''add'' riboswitch with stem III of wildtype hammerhead ribozyme by a selected linker of twelve basepairs.
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===Source===
 
===Source===
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The adenine aptamer was first found in ''V. vulnificus'' and the fast-cleaving hammerhead ribozyme is from ''Schistosoma mansonii''
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add an adenine responced aptamer on a wildtype hammerhead ribozyme
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===References===
 
===References===
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Maumita Mandal & Ronald R Breaker.(2004)Adenine riboswitches and gene activation by disruption of a transcription terminator. Nature structural& molecular biology
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Jörg S. Hartig et al. (2008) Artificial Ribozyme Switches Containing Natural Riboswitch Aptamer Domains. Angew. Chem. 121: 2753 –2756

Latest revision as of 06:21, 5 October 2011

pBAD+Adenine Hammerhead Ribozyme+RBS+E0040+B0015


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 919


Design Notes

This part constructs by connecting adenine aptamer from add riboswitch with stem III of wildtype hammerhead ribozyme by a selected linker of twelve basepairs.

Source

The adenine aptamer was first found in V. vulnificus and the fast-cleaving hammerhead ribozyme is from Schistosoma mansonii

References

Maumita Mandal & Ronald R Breaker.(2004)Adenine riboswitches and gene activation by disruption of a transcription terminator. Nature structural& molecular biology Jörg S. Hartig et al. (2008) Artificial Ribozyme Switches Containing Natural Riboswitch Aptamer Domains. Angew. Chem. 121: 2753 –2756