Difference between revisions of "Part:BBa K678000:Design"

(→‎Design Notes)
(→‎Design Notes)
 
(One intermediate revision by the same user not shown)
Line 6: Line 6:
  
 
===Design Notes===
 
===Design Notes===
−
This part was designed for the Plug'n'Play with DNA assembly standard (RFC 80), therefore this part contains linkers at the 5' and 3' ends as shown on the figure below.
+
This part was designed for the Plug 'n' Play with DNA assembly standard (RFC 80), therefore this part contains linkers at the 5' and 3' ends as shown on the figure below.
  
−
https://parts.igem.org/Image:Promotor_med_haler.png
+
[[Image:Promotor_med_haler.png|left|250px|caption|<b>DTU-Denmark-2 2011</b>]]
 +
<br clear=all>
  
−
This part was USER cloned into the shipping plasmid pSB1C3 but is also contained in the Plug'n'Play plasmid [https://parts.igem.org/wiki/index.php?title=Part:BBa_K678066 pJEJAM18]
+
This part can easily be fused with other parts adapted to the Plug 'n' Play assembly standard as shown below.
 +
 
 +
 
 +
[[Image:Overveiw_module_tails_2.png|left|450px|caption|<b>DTU-Denmark-2 2011</b>]]
 +
<br clear=all>
 +
 
 +
This part was USER cloned into the shipping plasmid pSB1C3 but is also contained in the Plug 'n' Play plasmid [https://parts.igem.org/wiki/index.php?title=Part:BBa_K678066 pJEJAM18]
  
 
===Source===
 
===Source===

Latest revision as of 06:46, 27 September 2011

DMKP-P6, Aspergillus nidulans promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 23
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 23
    Illegal NheI site found at 473
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 23
    Illegal XhoI site found at 122
    Illegal XhoI site found at 132
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 23
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 23
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 212
    Illegal BsaI.rc site found at 499


Design Notes

This part was designed for the Plug 'n' Play with DNA assembly standard (RFC 80), therefore this part contains linkers at the 5' and 3' ends as shown on the figure below.

DTU-Denmark-2 2011


This part can easily be fused with other parts adapted to the Plug 'n' Play assembly standard as shown below.


DTU-Denmark-2 2011


This part was USER cloned into the shipping plasmid pSB1C3 but is also contained in the Plug 'n' Play plasmid pJEJAM18

Source

Aspergillus nidulans genomic DNA (AN1122)

References

Dorte Koefoed Holm, Center for Microbial Biotechnology, Technical University of Denmark (Unpublished data).