Difference between revisions of "Part:BBa K644000"
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* This E-cadherin only contains the extracellular domains of the protein. | * This E-cadherin only contains the extracellular domains of the protein. | ||
| − | We cloned E-cadherin into our plasmid pCTCON2 because pCTCON2 contained the Aga1 and Aga2 protein gene sequences which helped display our E-cadherin and other cloned proteins on the surface of ''S. cerevisiae''. | + | We cloned E-cadherin into our plasmid pCTCON2 because pCTCON2 contained the Aga1 and Aga2 protein gene sequences which helped display our E-cadherin and other cloned proteins on the surface of ''S. cerevisiae''. pCTCON2 also contains a Galactose promoter and our proteins were only displayed when ''S. cerevisiae'' was grown under the presence of galactose. |
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| + | == E-cadherin expressed on ''S. cerevisiae'' with Calcium ions == | ||
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| + | *all the pictures are of EBY100 ''S. cerevisiae'' transformed with E-cadherin grown under the presence of galactose. | ||
[[Image:Cadherin+Ca.jpg]] | [[Image:Cadherin+Ca.jpg]] | ||
[[Image:Cadherin+Ca (1).jpg]] | [[Image:Cadherin+Ca (1).jpg]] | ||
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| + | == E-cadherin expressed on ''S. cerevisiae'' without Calcium == | ||
| + | [[Image:Cadherin.jpg]] | ||
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Latest revision as of 01:23, 27 September 2011
Extracellular Domain of E-Cadherin (Mouse)
Cadherin is an adhesive transmembrane and calcium dependent protein that is expressed in many mammalian tissue cells and plays a role in keeping tissues together. It has a domain that anchors the protein to the cytoskeleton of the mammalian cells, an intercellular domain, and five extracellular domain repeats. With the presence of calcium ions, the extracellalur domains become rigid and are able to form homodimers with the same type of cadherin or form heterodimers with different types of cadherin, hence creating cell to cell adhesion.
- This E-cadherin only contains the extracellular domains of the protein.
We cloned E-cadherin into our plasmid pCTCON2 because pCTCON2 contained the Aga1 and Aga2 protein gene sequences which helped display our E-cadherin and other cloned proteins on the surface of S. cerevisiae. pCTCON2 also contains a Galactose promoter and our proteins were only displayed when S. cerevisiae was grown under the presence of galactose.
E-cadherin expressed on S. cerevisiae with Calcium ions
- all the pictures are of EBY100 S. cerevisiae transformed with E-cadherin grown under the presence of galactose.
.jpg)
E-cadherin expressed on S. cerevisiae without Calcium
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 285
Illegal BamHI site found at 361
Illegal BamHI site found at 477
Illegal BamHI site found at 1401
Illegal BamHI site found at 1703
Illegal XhoI site found at 1085 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 405
Illegal BsaI site found at 947