Difference between revisions of "Part:BBa K566017"
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Red Fluorescent Protein under the control of pRM434 promoter. It was constructed for demonstrating dual state of a biphasic switch. | Red Fluorescent Protein under the control of pRM434 promoter. It was constructed for demonstrating dual state of a biphasic switch. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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+ | Homotretameric protein from Discosoma sp. Thought to play a role in photoprotection of the coral's resident symbiont microalgae's photosystems from photoinhibition caused by high light levels found near the surface of coral reefs. In deeper water, the fluorescence may be to convert blue light into longer wavelengths more suitable for use in photosynthesis by the microalgal symbionts. It belongs to the GFP family. It possesses bright fluorescence with excitation/emission maxima at 558 and 583 nm, respectively. It has an extinction coefficient of 72,500 M-1 cm-1, which means how strongly a chemical species absorbs light at a given wavelength. It has high absortion and high quantum yield (68%). The fluorescence quantum yield gives the efficiency of the fluorescence process. It is defined as the ratio of the number of photons emitted to the number of photons absorbed. Modified with a LVA tag for rapid degradation of the protein and faster fall time for the emission. | ||
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+ | [[Image:RFP.png|thumb|center|600px|Figure 1. Relative fluorescence of RFP (Ds-Red). Fluorescence excitation/emission maxima at 558 and 583 nm, respectively. | ||
+ | Figure taken and modified from Patterson G, Rich N Day and David P (2001) Fluorescent protein spectra. <i>Cell Science at a Glance</i> <b>5</b>:837-838 .]] | ||
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Latest revision as of 23:23, 28 September 2011
pRM434-RFP
Red Fluorescent Protein under the control of pRM434 promoter. It was constructed for demonstrating dual state of a biphasic switch.
Usage and Biology
Homotretameric protein from Discosoma sp. Thought to play a role in photoprotection of the coral's resident symbiont microalgae's photosystems from photoinhibition caused by high light levels found near the surface of coral reefs. In deeper water, the fluorescence may be to convert blue light into longer wavelengths more suitable for use in photosynthesis by the microalgal symbionts. It belongs to the GFP family. It possesses bright fluorescence with excitation/emission maxima at 558 and 583 nm, respectively. It has an extinction coefficient of 72,500 M-1 cm-1, which means how strongly a chemical species absorbs light at a given wavelength. It has high absortion and high quantum yield (68%). The fluorescence quantum yield gives the efficiency of the fluorescence process. It is defined as the ratio of the number of photons emitted to the number of photons absorbed. Modified with a LVA tag for rapid degradation of the protein and faster fall time for the emission.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 681
Illegal AgeI site found at 793 - 1000COMPATIBLE WITH RFC[1000]