Difference between revisions of "Part:BBa K615003:Design"
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<partinfo>BBa_K615003 short</partinfo> | <partinfo>BBa_K615003 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
rpoZ knocked out using lambda red | rpoZ knocked out using lambda red | ||
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Kanamycin cassette-Zif268 binding site-weak promoter placed upstream of endogenous TolC | Kanamycin cassette-Zif268 binding site-weak promoter placed upstream of endogenous TolC | ||
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===Source=== | ===Source=== | ||
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===References=== | ===References=== | ||
| + | Harris H. Wang, Farren J. Isaacs, Peter A. Carr, Zachary Z. Sun, George Xu, Craig R. Forest, George M. Church. Programming cells by multiplex genome engineering and accelerated evolution. (2009). Nature, 460(7257):894-8. | ||
Latest revision as of 03:47, 27 September 2011
E. coli strain for TolC one-hybrid selection system
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 632
Illegal PstI site found at 952 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 632
Illegal PstI site found at 952 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 845
Illegal BglII site found at 965 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 632
Illegal PstI site found at 952 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 632
Illegal PstI site found at 952
Illegal NgoMIV site found at 181 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 121
Illegal SapI site found at 331
Design Notes
rpoZ knocked out using lambda red
Kanamycin cassette-Zif268 binding site-weak promoter placed upstream of endogenous TolC
Source
Based off EcNR2 selection strain (see Wang et al, 2009)
References
Harris H. Wang, Farren J. Isaacs, Peter A. Carr, Zachary Z. Sun, George Xu, Craig R. Forest, George M. Church. Programming cells by multiplex genome engineering and accelerated evolution. (2009). Nature, 460(7257):894-8.