Difference between revisions of "Part:BBa K634007:Experience"

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how you used this part and how it worked out.
 
how you used this part and how it worked out.
  
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===Applications of BBa_K634007===
 
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When placed 3' of the promoter portion of a 1- or 2-component sensor, BBa_634007 can be used in the following ways:
 
  
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Reporter: tagRFP (BBa_K634005) fluoresces red upon induction (see BBa_K634005 for issues with fluorescence)
 
  
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Selection: Sensor systems can be selected for desired induced expression through supplementation of the culture with the sensor's analyte and kanamycin.  
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== Issues in practice ==
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''sacB'' appears to be something of a lightning rod for loss of function mutations. As can be seen above, sucrose lethality is only maintained in a population for approximately 5 hours. At this point, we presume that spontaneous mutations in ''sacB'' produce sucrose-insensitive mutants.
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Counter selection: Sensors can also be selected for absence of expression when uninduced through supplementation of the culture with sucrose
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===User Reviews===
 
===User Reviews===

Latest revision as of 03:48, 28 September 2011

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.


Issues in practice

sacB appears to be something of a lightning rod for loss of function mutations. As can be seen above, sucrose lethality is only maintained in a population for approximately 5 hours. At this point, we presume that spontaneous mutations in sacB produce sucrose-insensitive mutants.

User Reviews

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