Difference between revisions of "Part:BBa K566014"

 
 
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<partinfo>BBa_K566014 short</partinfo>
 
<partinfo>BBa_K566014 short</partinfo>
  
Composite part for Biphasic switch tests.
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Composite part for Biphasic switch tests. Include pRM promoter from phage λ pRM promoter from Lambda phage. It may be positively and negatively regulated according to cI protein concentration. Low [cI] turn it ON, high [cI] turn it OFF. It contains three operators named OR1, OR2 and OR3, all cI binding sites. When bound to OR1 and OR2, cI positively regulates pRM; cI binding to OR3 repress pRM. However, due to a low binding affinity to OR3, higher concentrations of the protein are required.
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A medium RBS sequence, strength is considered relative to Strong (<bbpart>BBa_B0030</bbpart>), Weak (<bbpart>BBa_B0031</bbpart>) and weaker (<bbpart>BBa_B0033</bbpart>). Also contain the cI434 coding sequence optimized for ''E. coli'', its come from the registered part cI434 repressor protein (<bbpart>BBa_C0052</bbpart>), It binds to the 434 regulatory sequence in λ pRM promoter. The sequence contains a LVA tag for faster degradation.
  
  

Latest revision as of 05:06, 28 September 2011

pRM-RBS-cI434

Composite part for Biphasic switch tests. Include pRM promoter from phage λ pRM promoter from Lambda phage. It may be positively and negatively regulated according to cI protein concentration. Low [cI] turn it ON, high [cI] turn it OFF. It contains three operators named OR1, OR2 and OR3, all cI binding sites. When bound to OR1 and OR2, cI positively regulates pRM; cI binding to OR3 repress pRM. However, due to a low binding affinity to OR3, higher concentrations of the protein are required.

A medium RBS sequence, strength is considered relative to Strong (BBa_B0030), Weak (BBa_B0031) and weaker (BBa_B0033). Also contain the cI434 coding sequence optimized for E. coli, its come from the registered part cI434 repressor protein (BBa_C0052), It binds to the 434 regulatory sequence in λ pRM promoter. The sequence contains a LVA tag for faster degradation.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 534
    Illegal AgeI site found at 545
  • 1000
    COMPATIBLE WITH RFC[1000]