Difference between revisions of "Part:BBa K566010"

Latest revision as of 07:12, 28 September 2011

PenI repressor optimized for E. coli (inverted sequence)

Optimized PenI repressor with LVA tag from Bacillus licheniformis, with preferential codon usage for improved expression in E. coli. Negatively regulates transcription from pPenI promoter (BBa_R0074). The nucleotide sequence was inverted to ease DNA synthesis.

Usage and Biology

The penicillinase repressor (PenI) negatively regulates expression of the penicillinase gene (penP) in Bacillus licheniformis by binding to its operators located within the promoter region of penP. penI codes for a protein with 128 amino acids. Filter-binding analyses suggest that the active form of the repressor is a dimer.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K566010 short</partinfo>
-PenI repressor with codon optimization for improving expression into E. coli.
+Optimized PenI repressor with LVA tag from ''Bacillus licheniformis'', with preferential codon usage for improved expression in <i>E. coli</i>. Negatively regulates transcription from pPenI promoter ([https://parts.igem.org/Part:BBa_R0074 BBa_R0074]). The nucleotide sequence was inverted to ease DNA synthesis.
-This protein represents a negative repressor for pPenI promoter (BBa_R0074).
-Sequence was inverted for simplifying DNA synthesis.
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+The penicillinase repressor (PenI) negatively regulates expression of the penicillinase gene (penP) in <i>Bacillus licheniformis</i> by binding to its operators located within the promoter region of penP. penI codes for a protein with 128 amino acids. Filter-binding analyses suggest that the active form of the repressor is a dimer.
 <!-- -->