Difference between revisions of "Part:BBa K566007:Design"

 
 
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===References===
 
===References===
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<b>1.</b> Wittman V, Lin HC, Wong HC (1993) Functional domains of the penicillinase repressor of <i>Bacillus licheniformis</i>. <i>J Bacteriol</i> <b>175</b>:7383-7390

Latest revision as of 06:02, 28 September 2011

PenI repressor optimized for E. coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Search for illegal sites was performed, they were found and eliminated.


Source

Original sequence was taken from Registry (BBa_C0074), this was optimized with preferential codons for better expression in E. coli.

References

1. Wittman V, Lin HC, Wong HC (1993) Functional domains of the penicillinase repressor of Bacillus licheniformis. J Bacteriol 175:7383-7390