Difference between revisions of "Part:BBa K566003:Design"

 
 
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__NOTOC__
 
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<partinfo>BBa_K566003 short</partinfo>
 
<partinfo>BBa_K566003 short</partinfo>
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===Design Notes===
 
===Design Notes===
Preferential codons were implemented for improving expression in E. coli.  
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Preferential codons were implemented for improving expression in ''E. coli''.  
  
 
Analysis of illegal sites was made, they were found and removed.
 
Analysis of illegal sites was made, they were found and removed.
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Synthesized DNA.
 
Synthesized DNA.
  
===References===
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==References==
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<b> 1.</b> Patterson G, Rich N Day and David P (2001) Fluorescent protein spectra. <i>Cell Science at a Glance</i> <b>5</b>:837-838

Latest revision as of 18:56, 28 September 2011

CFP optimized for E. coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 28
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Preferential codons were implemented for improving expression in E. coli.

Analysis of illegal sites was made, they were found and removed.


Source

Synthesized DNA.

References

1. Patterson G, Rich N Day and David P (2001) Fluorescent protein spectra. Cell Science at a Glance 5:837-838