Difference between revisions of "Part:BBa K530028"

Latest revision as of 01:41, 25 September 2011

pRSBB403 HIS3 Integrating Yeast Shuttle Vector

Yeast shuttle vector for propagation in E. coli and transformation into S. cerevisiae. Contains an Ampicillin resistance marker for E. coli and HIS3 selectable marker for S. cerevisiae. Fully compatible for use with Standard Assembly. Contains EcoRI, XbaI, SpeI, and PstI in the multiple cloning site, in that order. This is chromosomal integrating vector.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1008
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 899
Illegal BglII site found at 959
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1748
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 3428
Illegal SapI site found at 2345

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 <partinfo>BBa_K530028 short</partinfo>
-Yeast shuttle vector for propagation in ''E. coli'' and transformation into ''S. cerevisiae''. Contains an Ampicillin resistance marker for ''E. coli'' and HIS3 selectable marker for ''S. cerevisiae''. Fully compatible for use with Standard Assembly. Contains EcoRI, XbaI, SpeI, and PstI in the multiple cloning site, in that order.
+Yeast shuttle vector for propagation in ''E. coli'' and transformation into ''S. cerevisiae''. Contains an Ampicillin resistance marker for ''E. coli'' and HIS3 selectable marker for ''S. cerevisiae''. Fully compatible for use with Standard Assembly. Contains EcoRI, XbaI, SpeI, and PstI in the multiple cloning site, in that order. This is chromosomal integrating vector.
 <!-- Add more about the biology of this part here
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 <partinfo>BBa_K530028 parameters</partinfo>
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+===Plasmid Map===
+[[Image:pRSBB403.png]]