Difference between revisions of "Part:BBa K530038"

Latest revision as of 01:15, 25 September 2011

pRSBB425 LEU2 2Micron Episomal Yeast Shuttle Vector

Yeast shuttle vector for propagation in E. coli and transformation into S. cerevisiae. Contains an Ampicillin resistance marker for E. coli and LEU2 selectable marker for S. cerevisiae. Contains EcoRI, NheI, SpeI, and PstI in the multiple cloning site, in that order. For assembly, this vector differs from a Standard Assembly vector by replacing XbaI with NheI. This vector is a 2micron episomal yeast vector.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 1116
Illegal XbaI site found at 6309
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 1116
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 1116
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 1116
Illegal XbaI site found at 6309
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 1116
Illegal XbaI site found at 6309
Illegal NgoMIV site found at 2799
Illegal AgeI site found at 1503
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 4479
Illegal SapI site found at 3396

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 __NOTOC__
 <partinfo>BBa_K530038 short</partinfo>
-LEU2
+Yeast shuttle vector for propagation in ''E. coli'' and transformation into ''S. cerevisiae''. Contains an Ampicillin resistance marker for ''E. coli'' and LEU2 selectable marker for ''S. cerevisiae''.  Contains EcoRI, NheI, SpeI, and PstI in the multiple cloning site, in that order. For assembly, this vector differs from a Standard Assembly vector by replacing XbaI with NheI. This vector is a 2micron episomal yeast vector.
 <!-- Add more about the biology of this part here
@@ Line 17: / Line 16: @@
 <partinfo>BBa_K530038 parameters</partinfo>
 <!-- -->
+===Plasmid Map===
+[[Image:pRS425.png]]