Difference between revisions of "Part:BBa K575028"
 
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<partinfo>BBa_K575028 short</partinfo>
 
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Continuous expression of LasR (with RBS B0034), coupled with a LasR/PAI1 (3-oxo-C12-HSL) inducible promoter, RBS (Part B0030), and a GFP reporter.
 
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<!-- Add more about the biology of this part here
 
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===Usage and Biology===
 
===Usage and Biology===
 
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This construct is designed to report the presence of PAI1, part of the ''Pseudomonas'' Quorum sensing hierarchy. When induced with PAI-1 (3-oxo-C12-HSL) at varying concentrations, this construct fluoresced more than the control. Which allows us to use this part as a biosensor for the detection of Pseudomonas Aeruginosa.
 
[[Image:Standardized Fluorescence for S2.jpg]]__NOTOC__
 
[[Image:Standardized Fluorescence for S2.jpg]]__NOTOC__
  
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<partinfo>BBa_K575028 short</partinfo>
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Continuous expression of LasR (with RBS B0034), coupled with a LasR/PAI1 (3-oxo-C12-HSL) inducible promoter, RBS (Part B0030), and a GFP reporter.
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K575028 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K575028 SequenceAndFeatures</partinfo>
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<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Latest revision as of 01:04, 26 September 2011

This construct is designed to report the presence of PAI1, part of the Pseudomonas Quorum sensing hierarchy. When induced with PAI-1 (3-oxo-C12-HSL) at varying concentrations, this construct fluoresced more than the control. Which allows us to use this part as a biosensor for the detection of Pseudomonas Aeruginosa. Standardized Fluorescence for S2.jpg


LasR/PAI1 Inducible Promoter + RBS (B0034) + GFP, Constitutive Promoter + RBS (B0030) + LasR

Continuous expression of LasR (with RBS B0034), coupled with a LasR/PAI1 (3-oxo-C12-HSL) inducible promoter, RBS (Part B0030), and a GFP reporter. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 58
    Illegal NheI site found at 918
    Illegal NheI site found at 941
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1297
    Illegal AgeI site found at 1494
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 827