Difference between revisions of "Part:BBa K590031"
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<partinfo>BBa_K590031 short</partinfo> | <partinfo>BBa_K590031 short</partinfo> | ||
| − | This part encodes an enzyme | + | This part encodes an enzyme Aldehyde Decarbonylase (ADC) that removes the carbonyl group (C=O) from a fatty aldehyde, yielding an alkane one carbon shorter than the original aldehyde and a molecule of formate. Works together with [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590032 Acyl-ACP Reductase] (AAR) to produce odd chain length alkanes. This part is an important component of the [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590025 PetroBrick], which generates alkanes using AAR and ADC. |
[[Image:Fatty_Acid_Biosynthesis.jpg#file |250px]] | [[Image:Fatty_Acid_Biosynthesis.jpg#file |250px]] | ||
Latest revision as of 18:43, 23 September 2011
Aldehyde Decarbonylase (ADC) - Synechococcus elongatus PCC7942
This part encodes an enzyme Aldehyde Decarbonylase (ADC) that removes the carbonyl group (C=O) from a fatty aldehyde, yielding an alkane one carbon shorter than the original aldehyde and a molecule of formate. Works together with Acyl-ACP Reductase (AAR) to produce odd chain length alkanes. This part is an important component of the PetroBrick, which generates alkanes using AAR and ADC.
Usage and Biology
When AAR and ADC are co-expressed, they produce odd chain length alkanes from the cell's fatty acid biosynthetic pathway. Alkane production is enhanced when growing expression strains using the growth conditions developed by the [http://2011.igem.org/Team:Washington 2011 University of Washington iGEM Team].
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 730
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]