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| | <partinfo>BBa_K660201 short</partinfo> | | <partinfo>BBa_K660201 short</partinfo> |
| − | <h3>Origin</h3>
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| − | Horse sweat <i>(Equus caballus)</i><br/>
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| − | <h3>Function</h3>
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| − | Latherin is a surfactant protein with detergent like properties. <br/>
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| − | Its normal biological function is temperature regulation and is believed to function by enhancing evaporation from the pelt. It does so by binding to hydrophobic surfaces and allowing them to become wettable. <Br/>
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| − | <h3>Applications</h3>
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| − | Latherin is able to temporarily bind to hydrophobic surfaces and has therefore been investigated as a possible method of breaking up biofilms. It is also suspected that the protein may have antimicrobial functions. <br/>
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| − | The 2011 University of Glasgow iGEM team used this part in their system, DISColi, to aid in the dispersal of biofilms.
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| − | <h3>Additional Information</h3>
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| − | Note: Latherin is suspected to be the cause of allergic reaction in individuals with an allergy to horses.<br/>
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| − | <h3>Illegal Restriction Sites</h3>
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| − | We are aware of two illegal restriction sites that exist in our latherin biobrick. Due to time constraints, we were unable to complete site directed mutagenesis. The primers we designed for this are below, for any future team.<br/><br/>
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| − | <u>1st PstI site:</u><br/>
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| − | 5': GCG GAA ATT AAA CTG CAA GAT ACC CGT CTG CAA CTG<br/><br/>
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| − |     Forward Primer: 5' CAG TTG CAG ACG GGT ATC TTG CAG TTT AAT TTC CGC<br/><br/>
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| − |     Reverse Primer: 5' GCG GAA ATT AAA CTG CAA GAT ACC CGT CTG CAA CTG<br/><br/>
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| − | MTP= 79.33 <br/><br/>
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| − |
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| − | <u>2nd PstI site:</u><br/>
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| − | 5': GCG ATT GAG CTG CAA AGC GGC AAT CCG C<br/><br/>
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| − |
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| − |     Forward Primer: 5' G CGG ATT GCC GCT TTG CAG CTC AAT CGC<br/><br/>
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| − |
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| − |     Reverse Primer: 5' GCG ATT GAG CTG CAA AGC GGC AAT CCG C<br/><br/>
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| − | MTP= 78.71<br/><br/>
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| − | <h3>References & Further Reading</h3>
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| − | Beegley J, et al., 1986. Isolation and characterization of latherin, a surface-active protein from horse sweat<br/>http://www.ncbi.nlm.nih.gov/sites/entrez?db=pubmed&cmd=search&term=3753435>.
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| − | <br/><br/>
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| − | Kennedy M., 2011., Latherin and other biocompatible surfactant proteins<br/>
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| − | http://www.biochemsoctrans.org/bst/039/bst0391017.htm
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| − | <partinfo>BBa_K660201 SequenceAndFeatures</partinfo>
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