Difference between revisions of "Part:BBa K608102"
| (2 intermediate revisions by one other user not shown) | |||
| Line 7: | Line 7: | ||
It functions as the receptor for green light and is made up of a N-terminal transmembrane helix, a cyanobactreria specific GAF domain, two PAS domains and a C-terminal histidine kinase. To be fully functional CcaS has to bind the chromophore Phycocyanobilin (PCB) with its GAF-domain. The GAF domain in this system has the ligation motif Cys-Leu, instead of the usual plant GAF-domain with Cys-His | It functions as the receptor for green light and is made up of a N-terminal transmembrane helix, a cyanobactreria specific GAF domain, two PAS domains and a C-terminal histidine kinase. To be fully functional CcaS has to bind the chromophore Phycocyanobilin (PCB) with its GAF-domain. The GAF domain in this system has the ligation motif Cys-Leu, instead of the usual plant GAF-domain with Cys-His | ||
| + | [[Image:FR2011 Green light system.jpg |350px]] | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
| − | + | The natural sequence has an internal EcoRI and SpeI restriction site.<br/> | |
| + | We tried to delete these sites by PCR, but where not completely successful.<br/> | ||
| + | You can use this part in order to assemble the green light receptor system. <br/> | ||
| Line 16: | Line 19: | ||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K608102 SequenceAndFeatures</partinfo> | <partinfo>BBa_K608102 SequenceAndFeatures</partinfo> | ||
| + | ===Improvement=== | ||
| + | Designed by: Du liaoqi Group: iGEM18_WHU-China (2018-10-05) <br/> | ||
| + | We have condon-optimeized Ccas BBa_K2789009 for E.coli from BBa_K608102 and deleted the EcoRI and SpeI site in the gene sequence.<br/> | ||
| + | (https://parts.igem.org/Part:BBa_K2789001:Design)<br/> | ||
Latest revision as of 11:44, 17 October 2018
CcaS, green light receptor
CcaS is a cyanobacteriochrome, which is known to chromatically regulate the expression of the phycobilisome linker gene (cpcG2) in Synechocystis (see references). It functions as the receptor for green light and is made up of a N-terminal transmembrane helix, a cyanobactreria specific GAF domain, two PAS domains and a C-terminal histidine kinase. To be fully functional CcaS has to bind the chromophore Phycocyanobilin (PCB) with its GAF-domain. The GAF domain in this system has the ligation motif Cys-Leu, instead of the usual plant GAF-domain with Cys-His
Usage and Biology
The natural sequence has an internal EcoRI and SpeI restriction site.
We tried to delete these sites by PCR, but where not completely successful.
You can use this part in order to assemble the green light receptor system.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1685
Illegal SpeI site found at 1780 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1685
Illegal NheI site found at 1056
Illegal SpeI site found at 1780 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1685
Illegal XhoI site found at 6 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1685
Illegal SpeI site found at 1780 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1685
Illegal SpeI site found at 1780
Illegal AgeI site found at 1720 - 1000COMPATIBLE WITH RFC[1000]
Improvement
Designed by: Du liaoqi Group: iGEM18_WHU-China (2018-10-05)
We have condon-optimeized Ccas BBa_K2789009 for E.coli from BBa_K608102 and deleted the EcoRI and SpeI site in the gene sequence.
(https://parts.igem.org/Part:BBa_K2789001:Design)
References
Yuu Hirose et al ( 2008 )"Cyanobacteriochrome CcaS is the green light receptor that induces the expression of phycobilisome linker protein." Proc Natl Acad Sci U S A. 105: 9528–9533.
Tabor, J. J. et al.(2010), " Multichromatic Control of Gene Expression in Escherichia coli", J. Mol. Biol. , doi:10.1016/j.jmb.2010.10.038