Difference between revisions of "Part:BBa K607024"
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LasR protein with LVA degradation tag under transcriptional control of PlasB promoter and reverse antisense pBad/araC promoter, the RBS has a strength of 0.3 (obtained by Jason Kelly and Robbie Bryant during the summer of 2004 using a fluorescent reporter). This construct represents (in presence of N-(3-oxododecanoyl)homoserine lactone in the media) an auto-inducing loop in which the PlasB promoter drives expression of its own inducer LasR. The running loop can be turned off through activation of the reverse antisense PBad promoter by arabinose. | LasR protein with LVA degradation tag under transcriptional control of PlasB promoter and reverse antisense pBad/araC promoter, the RBS has a strength of 0.3 (obtained by Jason Kelly and Robbie Bryant during the summer of 2004 using a fluorescent reporter). This construct represents (in presence of N-(3-oxododecanoyl)homoserine lactone in the media) an auto-inducing loop in which the PlasB promoter drives expression of its own inducer LasR. The running loop can be turned off through activation of the reverse antisense PBad promoter by arabinose. | ||
| − | [[Image: | + | [[Image:LasR2.png|Caption test]] |
[[Image:lciaulegend.png|frameless]] | [[Image:lciaulegend.png|frameless]] | ||
Latest revision as of 23:53, 21 September 2011
PlasB_LasR-LVA_antipBad/araC with 0.3RBS
This part is a member of LasR autoinducing loop family.
LasR protein with LVA degradation tag under transcriptional control of PlasB promoter and reverse antisense pBad/araC promoter, the RBS has a strength of 0.3 (obtained by Jason Kelly and Robbie Bryant during the summer of 2004 using a fluorescent reporter). This construct represents (in presence of N-(3-oxododecanoyl)homoserine lactone in the media) an auto-inducing loop in which the PlasB promoter drives expression of its own inducer LasR. The running loop can be turned off through activation of the reverse antisense PBad promoter by arabinose.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 195
Illegal NheI site found at 1111 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1172
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 643
Illegal AgeI site found at 840
Illegal AgeI site found at 1337 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1354