Difference between revisions of "Part:BBa K515001"
 
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<partinfo>BBa_K515001 short</partinfo>
 
<partinfo>BBa_K515001 short</partinfo>
 
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<p>Indoleacetimide hydrolase catalyses the hydrolysis of indole-3-acetamide to indole-3-acetate and ammonia. The enzyme originates from the plant pathogen <i> Pseudomonas savastanoi </i> which produces the auxin, indole-3-acetic acid via a two step pathway. </p>
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<p>Indoleacetimide hydrolase catalyses the hydrolysis of indoleacetamide to indoleacetate and ammonia. The enzyme originates from the plant pathogen <i> Pseudomonas savastanoi </i> which produces the auxin, indole-3-acetic acid via a two step pathway. </p>
 
<p>This is one of the parts of our composite auxin expressing construct,  
 
<p>This is one of the parts of our composite auxin expressing construct,  
 
<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K515100" target="_blank">BBa_K515100</a>. </p>
 
<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K515100" target="_blank">BBa_K515100</a>. </p>
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<p><b>This BioBrick has been sequence verified.</p></b>
 
<p><b>This BioBrick has been sequence verified.</p></b>
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<p><b>For the full characterisation of the device, please refer to the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K515100">BBa_K515100</a> page.</b></p>
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==Contribution: NUDT_CHINA 2015==
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Author: Xinyuan Qiu
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Summary: We built a new part based on this part to extend its function.
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We designed a new part by removing the RBS from this part to extend its usage.
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====A new Bio-brick was designed based on this part====
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Primers used to build the new part:
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F-Prime: 5’- GGAATTCGCGGCCGCTTCTAGAGATGCGCGAAATG -3’
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R-Prime: 5’- GCGGGCGGCGGACTAGTCTTATTAGCCTTTTAACAC -3’
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For further information,
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See BBa_K1789001
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This part was sent to the registry.

Latest revision as of 16:38, 18 September 2015

IaaH - Indoleacetamide hydrolyase

Indoleacetimide hydrolase catalyses the hydrolysis of indoleacetamide to indoleacetate and ammonia. The enzyme originates from the plant pathogen Pseudomonas savastanoi which produces the auxin, indole-3-acetic acid via a two step pathway.

This is one of the parts of our composite auxin expressing construct, BBa_K515100.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1052
  • 1000
    COMPATIBLE WITH RFC[1000]


This BioBrick has been sequence verified.

For the full characterisation of the device, please refer to the BBa_K515100 page.



Contribution: NUDT_CHINA 2015

Author: Xinyuan Qiu

Summary: We built a new part based on this part to extend its function.

We designed a new part by removing the RBS from this part to extend its usage.

A new Bio-brick was designed based on this part

Primers used to build the new part:

F-Prime: 5’- GGAATTCGCGGCCGCTTCTAGAGATGCGCGAAATG -3’

R-Prime: 5’- GCGGGCGGCGGACTAGTCTTATTAGCCTTTTAACAC -3’


For further information,

See BBa_K1789001

This part was sent to the registry.