Difference between revisions of "Part:BBa K091104:Experience"

Latest revision as of 08:52, 27 September 2011

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Applications of BBa_K091104

User Reviews

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[http://2011.igem.org/Team:KULeuven K.U.Leuven iGEM 2011 Team]

Characterization by K.U.Leuven 2011 iGEM Team

We transformed Bba_K091104 (=pLac/Mnt hybrid) in DH5α-cells, followed by a minipreparation. After this we did a restriction digest with EcoRI and SpeI. Since the pSB1A2 vector is 2079bp and the Lac/Mnt hybrid promoter should be 87bp, we also expect a fragment of 87bp, one of 2079 and one of 2166bp. However, we see a fragment of 1000bp and one of ±2000bp.

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 <partinfo>BBa_K091104 AddReview 1</partinfo>
-<I>SarahV, KULeuven iGEM team 2011</I>
+<I><B>[http://2011.igem.org/Team:KULeuven K.U.Leuven iGEM 2011 Team]</B></I>
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-We transformed Bba_K091104 (=pLac/Mnt hybrid) in DH5α-cells, followed by a minipreparation. After this we did a restriction digest with EcoRI and SpeI.  Since the pSB1A2  vector is 2079bp and the Lac/Mnt hybrid promoter should be 87bp, we also expect a fragment of 87bp, one of 2079 and one of 2166bp.
+<u><b>Characterization by K.U.Leuven 2011 iGEM Team</b></u>
-However,  we see a fragment of 1000bp and of ±2000bp.
+We transformed Bba_K091104 (=pLac/Mnt hybrid) in DH5α-cells, followed by a minipreparation. After this we did a restriction digest with EcoRI and SpeI.  Since the pSB1A2 vector is 2079bp and the Lac/Mnt hybrid promoter should be 87bp, we also expect a fragment of 87bp, one of 2079 and one of 2166bp.
+However, we see a fragment of 1000bp and one of ±2000bp.
+[[Image:Restriction_1807.jpg|thumb|center|360px|]]
 |}
-[[Image:Restriction_1807.jpg]]
 <!-- DON'T DELETE --><partinfo>BBa_K091104 EndReviews</partinfo>